Development of a BALB/c 3T3 neutral red uptake cytotoxicity test using a mainstream cigarette smoke exposure system
نویسندگان
چکیده
منابع مشابه
Characterization of the Vitrocell® 24/48 in vitro aerosol exposure system using mainstream cigarette smoke
BACKGROUND Only a few exposure systems are presently available that enable cigarette smoke exposure of living cells at the air-liquid interface, of which one of the most versatile is the Vitrocell® system (Vitrocell® Systems GmbH). To assess its performance and optimize the exposure conditions, we characterized a Vitrocell® 24/48 system connected to a 30-port carousel smoking machine. The Vitro...
متن کاملEURL ECVAM Recommendation on the 3T3 Neutral Red Uptake Cytotoxicity Assay for Acute Oral Toxicity Testing
Acute oral toxicity is currently being assessed by a suite of refinement test methods based on thetraditional LD50 lethality test and is, besides skin sensitisation, the only remaining animal test requiredunder REACH Annex VII. In view of assessing the use of alternatives for this health endpoint, EURL ECVAMconducted a study on the 3T3 Neutral Red Uptake cytotoxicity test method add...
متن کاملAppendix C Table of Contents 1.0 STANDARD OPERATING PROCEDURE (SOP) FOR THE BALB/C 3T3 NEUTRAL RED UPTAKE CYTOTOXICITY TEST - A TEST FOR BASAL
1.0 STANDARD OPERATING PROCEDURE (SOP) FOR THE BALB/C 3T3 NEUTRAL RED UPTAKE CYTOTOXICITY TEST A TEST FOR BASAL CYTOTOXICITY....................... 3 1.1 Background and Introduction.................................................................................................. 3 1.2 Rationale.........................................................................................................
متن کاملA comparative assessment of cigarette smoke aerosols using an in vitro air–liquid interface cytotoxicity test
This study describes the evaluation of a modified air-liquid interface BALB/c 3T3 cytotoxicity method for the assessment of smoke aerosols in vitro. The functionality and applicability of this modified protocol was assessed by comparing the cytotoxicity profiles from eight different cigarettes. Three reference cigarettes, 1R5F, 3R4F and CORESTA Monitor 7 were used to put the data into perspecti...
متن کاملSafety Assessment of Mainstream Smoke of Herbal Cigarette
Owing to the increase in price of cigarettes in Korea, herbal cigarettes have received increasing attention as a non-smoking aid; however, its safety has hardly been studied. We analyzed some of the toxic components in the mainstream smoke of herbal cigarettes, performed a mutagenicity test on smoke condensates for safety assessment, and compared the results with the corresponding values of a g...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Research Notes
سال: 2014
ISSN: 1756-0500
DOI: 10.1186/1756-0500-7-367